Homework 2 Questions

Homework

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Database Questions
Somewhere out on the Internet is a database of restriction enzymes.
a. Where is it located? What is the URL for the database file that could be used with the GCG software?
b. What does a typical entry look like for the restriction enzyme file that is formatted for use with the MacVector program?
c. How is the database (formatted for MacVector) organized?
1. What is the delimiter between individual restriction enzyme entries? How does the computer (or you) know when the information from one restriction enzyme stops and another one starts?
2. Is this format similar to the format used by any other database? Which one?
Literature Search Questions
1) Select a protein and find the entries for this protein in the GenBank DNA database, the SwissProt database, and the PDB Protein database. List the attributes or features that are common to the databases and those which are unique to each.
2) How many secreted proteins have been discovered in humans? Explain what database you used, and what keywords you used to do the search.